Lekmine Fatima, Salti George I
Department of Surgical Oncology, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
J Surg Res. 2008 Sep;149(1):27-30. doi: 10.1016/j.jss.2007.07.018. Epub 2007 Aug 22.
Microphthalmia transcription factor (Mitf) is implicated as the master gene for survival of melanocytes, as well as a key transcription factor regulating the expression of major melanogenic proteins. We have shown that Mitf is a novel prognostic marker in patients with melanoma and that it plays a role in melanoma differentiation. Melanocyte-stimulating hormone up-regulates Mitf expression. Forskolin is a known alpha-melanocyte-stimulating hormone agonist. This study was undertaken to determine if forskolin induces differentiation in UISO-Mel-6 cell lines. Forskolin inhibits cell proliferation and increases the expression of Mitf and tyrosinase related protein-1. It also increases extracellular melanin production. In addition, we showed by flow cytometry that forskolin decrease the number of cells in S-phase without engaging the cells in apoptosis. Our results suggest that Forskolin induces differentiation in melanoma cells via Mitf pathway.
小眼畸形转录因子（Mitf）被认为是黑素细胞存活的主控基因，也是调节主要黑素生成蛋白表达的关键转录因子。我们已经表明，Mitf是黑色素瘤患者的一种新型预后标志物，并且它在黑色素瘤分化中起作用。促黑素细胞激素上调Mitf表达。福斯高林是一种已知的α-促黑素细胞激素激动剂。本研究旨在确定福斯高林是否能诱导UISO-Mel-6细胞系分化。福斯高林抑制细胞增殖，增加Mitf和酪氨酸酶相关蛋白-1的表达。它还增加细胞外黑色素的产生。此外，我们通过流式细胞术表明，福斯高林减少了S期细胞的数量，而不会使细胞发生凋亡。我们的结果表明，福斯高林通过Mitf途径诱导黑色素瘤细胞分化。
