Zhuravleva Z N
Institute of Biophysics, USSR Academy of Sciences, Puschino-on-Oka.
J Hirnforsch. 1991;32(4):511-24.
The ultrastructure of cellular composition of the graft/host interface and its permeability for the connecting nervous fibers was investigated in the heterotopic intracortical grafts of the dentate fascia. The areas of interface, where definite border between the graft and host tissues was detected at the semithin sections, were analyzed. Three types of the graft/host interface were distinguished: i) astrocytic; ii) ependymal; and iii) perivascular. The astrocytic interface was crossed by bundles of thin unmyelinated axons (similar to the dentate mossy fibers), by fascicles of thick myelinated axons (resembling those of the neocortex), and by dendrites. Ependymal sheath, which was overdeveloped in the grafted dentate tissue, was perforated by axonal and axo-dendritic bundles, but only if they were accompanied by fibrous astrocytic processes. Wide perivascular spaces of thick-walled interface blood capillaries, where processes of perivascular glia were intermingled with numerous basal laminae, were impermeable for the nervous fibers. The data show, that extensive exchange by the nervous fibers exits between dentate and surrounding neocortex, and that various types of glial cells have different roles in supporting, or preventing development of the graft/host nervous integration.
在齿状筋膜的异位皮质内移植中，研究了移植物/宿主界面细胞组成的超微结构及其对连接神经纤维的通透性。分析了在半薄切片中检测到移植物与宿主组织之间存在明确边界的界面区域。区分出三种类型的移植物/宿主界面：i）星形细胞型；ii）室管膜型；iii）血管周围型。薄的无髓轴突束（类似于齿状苔藓纤维）、厚的有髓轴突束（类似于新皮质的轴突束）和树突穿过星形细胞界面。移植的齿状组织中过度发育的室管膜鞘被轴突束和轴突-树突束穿孔，但前提是它们伴有纤维性星形细胞突起。厚壁界面毛细血管的宽阔血管周围间隙，其中血管周围神经胶质细胞的突起与众多基膜交织在一起，对神经纤维是不可渗透的。数据表明，齿状结构与周围新皮质之间存在广泛的神经纤维交换，并且各种类型的神经胶质细胞在支持或阻止移植物/宿主神经整合的发展中具有不同作用。
