Ganesh Krishna N, Gourishankar A, Vysabhattar Raman, Bokil Pradnya
National Chemical Laboratory, Pune, India.
Nucleic Acids Symp Ser (Oxf). 2007(51):17-8. doi: 10.1093/nass/nrm009.
We herein describe the introduction of gem-dimethyl substitution into the aminoethylglycyl backbone of PNA to impart steric constraint and pre-organise PNA for selective recognition of nucleic acids. Introduction of cyanuric acid and 8-aminoadenine as pyrimidine and purine analogs that can form base pairing from either face is also described to overcome the rotameric problems in PNA sidechain orientations and thereby enhance the statistical probability for base pairing. The UV-thermal melting studies of the derived triplexes with complementary DNA provide support for this rationale.
我们在此描述了将偕二甲基取代引入肽核酸（PNA）的氨乙基甘氨酰主链中，以施加空间位阻并使PNA预组织化，从而实现对核酸的选择性识别。还描述了引入氰尿酸和8-氨基腺嘌呤作为嘧啶和嘌呤类似物，它们可以从任一侧面形成碱基对，以克服PNA侧链取向中的旋转异构体问题，从而提高碱基配对的统计概率。对与互补DNA形成的三链体进行的紫外热变性研究为这一原理提供了支持。
