Yamamoto Junpei, Tanaka Yoshiyuki, Hitomi Kenichi, Getzoff Elizabeth D, Iwai Shigenori
Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
Nucleic Acids Symp Ser (Oxf). 2007(51):79-80. doi: 10.1093/nass/nrm040.
The (6-4) photoproduct, which is one of major UV-induced lesions formed between adjacent pyrimidine bases in DNA, was characterized by using (15)N NMR, fluorescence emission, and UV/VIS absorption at various pH values. From these experiments, we identified a novel intramolecular hydrogen bond between the hydroxyl group at the C5 position of the 5' component and the N3 of the 3' pyrimidone ring within the (6-4) photoproduct.
（6-4）光产物是DNA中相邻嘧啶碱基之间形成的主要紫外线诱导损伤之一，通过在不同pH值下使用（15）N核磁共振、荧光发射和紫外/可见吸收对其进行了表征。通过这些实验，我们在（6-4）光产物内5' 组分C5位置的羟基与3' 嘧啶酮环的N3之间鉴定出一种新型分子内氢键。
