Roongjang Somjing, Takahashi Kurena, Obika Satoshi, Imanishi Takeshi
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Nucleic Acids Symp Ser (Oxf). 2007(51):113-4. doi: 10.1093/nass/nrm057.
Recently, we described the antisense activity of 2',4'-BNA oligonucleotides in living cells.(1) Here, we examine the antisense effect of 2',4'-BNA, 2',4'-(COC) and 3'-amino-2',4'-BNA oligonucleotides targeting the bcl-xL gene. The results showed that while S-oligo had a slightly inhibiting effect on bcl-xL expression and natural DNA had no effect, an antisense oligonucleotide (AON) containing six 2',4'-(COC) nucleotides reduced the bcl-xL mRNA level to 64% of the untreated level. Interestingly, several types of AON-those containing nine 2',4'-BNA(COC) nucleotides, 2',4'-BNA and 3'-amino-2',4'-BNA- completely inhibited expression of the target gene. These data show that, compared with the corresponding natural DNA and S-oligo, BNA-based AONs are efficient inhibitors of bcl-xL expression.
最近，我们描述了2'，4'-BNA寡核苷酸在活细胞中的反义活性。（1）在此，我们研究了靶向bcl-xL基因的2'，4'-BNA、2'，4'-(COC)和3'-氨基-2'，4'-BNA寡核苷酸的反义效应。结果表明，虽然S-寡核苷酸对bcl-xL表达有轻微抑制作用，而天然DNA无作用，但含有六个2'，4'-(COC)核苷酸的反义寡核苷酸（AON）将bcl-xL mRNA水平降低至未处理水平的64%。有趣的是，几种类型的AON——含有九个2'，4'-BNA(COC)核苷酸、2'，4'-BNA和3'-氨基-2'，4'-BNA的AON——完全抑制了靶基因的表达。这些数据表明，与相应的天然DNA和S-寡核苷酸相比，基于BNA的AON是bcl-xL表达的有效抑制剂。
