利用锌指蛋白Sp2对嗜肺军团菌费城1型菌株的PCR产物进行特异性检测。

Specific detection of PCR product from Legionella pneumophila strain Philadelphia1 using zinc finger protein Sp2.

作者信息

Ikebukuro Kazunori, Kumagai Takenori, Motoki Hiroaki, Osawa Yuko, Matuo Takafumi, Horiuchi Michio, Sode Koji

机构信息

Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture & Technology, 2-24-16 Naka-machi, Koganei, Tokyo 184-8588, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2007(51):285-6. doi: 10.1093/nass/nrm143.

DOI:10.1093/nass/nrm143

PMID:18029698

Abstract

We have developed a novel method to detect PCR products from pathogen genome DNA by Zinc finger protein that can bind to double strand DNA (dsDNA) in sequence specific manner. In this study, we tried to detect Legionella pneumophila strain Philadelphia 1 using Zinc finger protein. We found the specific target DNA sequence for zinc finger protein Sp2 in L. pneumophila strain Philadelphia 1 genome DNA. Specific PCR product was successfully amplified from L. pneumophila strain Philadelphia 1 genome DNA and we used Zinc finger protein Sp2 to detect it. We succeeded in detecting the PCR products from L. pneumophila strain Philadelphia 1 genome DNA with Sp2.

摘要

我们开发了一种新方法，可通过能以序列特异性方式结合双链DNA（dsDNA）的锌指蛋白来检测病原体基因组DNA中的PCR产物。在本研究中，我们尝试使用锌指蛋白检测嗜肺军团菌费城1型菌株。我们在嗜肺军团菌费城1型菌株的基因组DNA中找到了锌指蛋白Sp2的特异性靶DNA序列。从嗜肺军团菌费城1型菌株的基因组DNA中成功扩增出特异性PCR产物，并且我们使用锌指蛋白Sp2对其进行检测。我们成功地用Sp2检测到了嗜肺军团菌费城1型菌株基因组DNA中的PCR产物。