用于恒河猴组织冰冻切片中免疫活性细胞免疫组织化学标记的单克隆抗体。
Monoclonal antibodies for immunohistochemical labeling of immunocompetent cells in frozen sections of rhesus monkey tissues.
作者信息
Stevens H P, van der Kwast T H, Timmermans A, Stouten N, Jonker M
机构信息
Department of Plastic and Reconstructive Surgery, University Hospital Dijkzigt, Rotterdam, The Netherlands.
出版信息
J Med Primatol. 1991 Oct;20(8):386-93.
Twenty-eight anti-human and two rhesus specific monoclonal antibodies (MAbs) were evaluated for immunohistochemical peroxidase staining reactivity in rhesus monkeys lymph nodes, thymus, liver, and skin allografts. Reactivity with the following antigens was assessed: MHC class I, II-DR, -DQ, and -DP antigens; leukocyte markers CD1, CD2, CD3, CD4, CD8, CD14, CD16, CD25, CD57; a proliferation associated nuclear antigen; interferon-gamma and tumor necrosis factor-alpha. Twenty-three MAbs proved to be suitable for immunohistochemical staining on frozen sections.
对28种抗人单克隆抗体和2种恒河猴特异性单克隆抗体（MAbs）进行了评估，以检测它们在恒河猴淋巴结、胸腺、肝脏和皮肤同种异体移植中的免疫组织化学过氧化物酶染色反应性。评估了它们与以下抗原的反应性：MHC I类、II-DR、-DQ和-DP抗原；白细胞标志物CD1、CD2、CD3、CD4、CD8、CD14、CD16、CD25、CD57；一种增殖相关核抗原；干扰素-γ和肿瘤坏死因子-α。23种单克隆抗体被证明适用于冷冻切片的免疫组织化学染色。
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