Buhler Rogerio Borghi, Sennes Luiz U, Mauad Thais, Melo Erich Christiano M, Silva Luiz Fernando F, Saldiva Paulo Hilário N
Department of Otolaryngology, the University of São Paulo School of Medicine, São Paulo, Brazil.
Laryngoscope. 2008 Feb;118(2):371-4. doi: 10.1097/MLG.0b013e318159aa0d.
To analyze the presence and distribution of collagen fibers and versican in human vocal fold lamina propria of fetal larynges.
Cross sectional analysis of cadaveric vocal folds of human fetuses.
Seven fetal larynges obtained from 28- to 36-week-old fetuses were analyzed with the Picrosirius-polarization method, immunohistochemistry, and image analysis.
Collagen fibers within the lamina propria exhibited a monolaminar distribution pattern and spatial arrangement in "wicker basket." Versican distribution was larger in the superficial and intermediate layers when compared to the deep layer.
Our findings suggest that collagen and versican distribution and arrangement within the lamina propria in the developing fetus are important for vocalization at birth.
分析人胎儿喉声带固有层中胶原纤维和多功能蛋白聚糖的存在及分布情况。
对人胎儿尸体声带进行横断面分析。
采用天狼星苦味酸偏振法、免疫组织化学和图像分析对7例取自28至36周龄胎儿的喉进行分析。
固有层内的胶原纤维呈现单层分布模式及“柳条篮”样空间排列。与深层相比，多功能蛋白聚糖在浅层和中层的分布更多。
我们的研究结果表明，发育中胎儿固有层内胶原和多功能蛋白聚糖的分布及排列对出生时发声很重要。
