猪心脏再灌注时通过冠状动脉内输注重组人超氧化物歧化酶未能缩小梗死面积：免疫组织化学和组织学分析

Failure to reduce infarct size by intracoronary infusion of recombinant human superoxide dismutase at reperfusion in the porcine heart: immunohistochemical and histological analysis.

作者信息

Matsuda M, Fujiwara H, Kawamura A, Ishida M, Takemura G, Kida M, Uegaito T, Fujiwara Y, Fujiwara T, Kawai C

机构信息

Department of Internal Medicine, Kyoto University, Japan.

出版信息

J Mol Cell Cardiol. 1991 Nov;23(11):1287-96. doi: 10.1016/0022-2828(91)90085-z.

DOI:10.1016/0022-2828(91)90085-z

PMID:1803019

Abstract

Failure to Reduce Infarct Size by Intracoronary Infusion of Recombinant Human Superoxide Dismutase at Reperfusion in the Porcine Heart: Immunohistochemical and Histological Analysis. Journal of Molecular and Cellular Cardiology (1991) 23, 1287-1296. We quantitatively determined the extent of infarction and contraction band necrosis in porcine hearts, and analyzed the distribution of administered recombinant human superoxide dismutase (h-SOD) in the myocardium using a polyclonal antibody to h-SOD. After 1 hour of occlusion, h-SOD was infused for the first 30 min of reperfusion in SOD group, while pigs received only arterial blood in control group. The extent of infarction or contraction band necrosis was not significantly different between SOD group and control group. Positive staining by polyclonal antibody to h-SOD was detected only in the infarcted area in SOD group. Thus, h-SOD only entered irreversibly damaged myocytes and neither diminished reperfusion injury nor reduced infarct size in pigs.

摘要

猪心脏再灌注时经冠状动脉输注重组人超氧化物歧化酶未能缩小梗死面积

免疫组织化学和组织学分析。《分子与细胞心脏病学杂志》（1991年）第23卷，第1287 - 1296页。我们定量测定了猪心脏梗死和收缩带坏死的程度，并使用抗人超氧化物歧化酶（h - SOD）的多克隆抗体分析了所给予的重组人超氧化物歧化酶（h - SOD）在心肌中的分布。闭塞1小时后，在SOD组再灌注的最初30分钟内输注h - SOD，而对照组猪只接受动脉血。SOD组和对照组之间梗死或收缩带坏死的程度无显著差异。在SOD组中，仅在梗死区域检测到抗h - SOD多克隆抗体的阳性染色。因此，h - SOD仅进入不可逆损伤的心肌细胞，在猪中既未减轻再灌注损伤也未缩小梗死面积。