[脑膜瘤中IGF-II相关蛋白的mRNA含量异常高]

[Exceptionally high content of mRNA of IGF-II-associated protein in meningiomas].

作者信息

Dmytrenko V V, Bukreieva T V, Shostak K O, Vitak N Ia, Pekars'kyĭ Iu A, Malysheva T A, Shamaiev M I, Rozumenko V D, Kondratiuk V V, Zozulia Iu P, Kavsan V M

出版信息

Ukr Biokhim Zh (1999). 2007 Mar-Apr;79(2):55-61.

PMID:18030751

Abstract

A comparison of gene expression profiles in different types of human brain tumours and normal brain by Serial Analysis of Gene Expression (SAGE) revealed exceptionally high content of CTTGGGTTTT tag in meningioma and ependimoma SAGE-libraries. A search of the most relevant gene for this tag on the website "SAGE Anatomic Viewer" showed that it belonged to the nucleotide sequence of insulin-like growth factor II (IGF-II) gene as well as to the open reading frame 43 on a chromosome 11 (C11orf43). This nucleotide sequence encodes putative insulin-like growth factor II associated protein (IGF-IIA). mRNA for this protein is produced as a result of the processing of IGF-II gene primary transcript. Northern analysis of glial tumours and meningiomas showed the exceptionally high level of mRNA of IGF-II-associated protein in meningiomas. Protein, encoded by this mRNA, can play the important role in meningioma formation and may be used as their specific molecular marker.

摘要

通过基因表达序列分析（SAGE）对不同类型人类脑肿瘤和正常脑组织中的基因表达谱进行比较，结果显示在脑膜瘤和室管膜瘤的SAGE文库中，CTTGGGTTTT标签的含量异常高。在“SAGE解剖学浏览器”网站上搜索与该标签最相关的基因，结果表明它属于胰岛素样生长因子II（IGF-II）基因的核苷酸序列，也属于11号染色体上的开放阅读框43（C11orf43）。该核苷酸序列编码假定的胰岛素样生长因子II相关蛋白（IGF-IIA）。这种蛋白质的mRNA是IGF-II基因初级转录本加工的产物。对胶质瘤和脑膜瘤进行的Northern分析表明，脑膜瘤中IGF-II相关蛋白的mRNA水平异常高。由该mRNA编码的蛋白质可能在脑膜瘤的形成中发挥重要作用，并可用作脑膜瘤的特异性分子标记。