Karakhim S O, Horchev V F, Kosterin S O
Ukr Biokhim Zh (1999). 2007 Mar-Apr;79(2):97-113.
Using equilibrium assumption the analysis of kinetics of enzyme/transport process has been developed. In the course of this process the enzyme can interact simultaneously with substrate S and effector E and as a result two products are generated: P1 (from substrate) and P2 (from effector) after catalytic effect of enzyme or transportation across the biological membrane. It has been demonstrated that the ratio (R = V0,1/V0,2) of initial rates of formation of reaction products P, (V0,1) and P2 (V0,2) represented in linearized form as a dependence on concentration of both substrate S0 and effector E0 allows to identify a specific mechanism of the present process. An algorithm of such identification has been developed.
利用平衡假设，已开展了酶/转运过程动力学分析。在此过程中，酶可同时与底物S和效应物E相互作用，结果产生两种产物：经酶的催化作用或跨生物膜转运后，来自底物的P1和来自效应物的P2。已证明，反应产物P1（V0,1）和P2（V0,2）初始形成速率的比值（R = V0,1/V0,2）以线性化形式表示为对底物S0和效应物E0浓度的依赖关系，这有助于确定当前过程的特定机制。已开发出一种进行此类识别的算法。
