Manásková P, Jonáková V
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
J Reprod Immunol. 2008 Jun;78(1):40-8. doi: 10.1016/j.jri.2007.10.001. Epub 2007 Nov 26.
Spermadhesins are proteins containing a characteristic CUB domain, originally isolated from seminal plasma and ejaculated spermatozoa in domestic animals. Boar spermadhesins are multifunctional proteins exhibiting ligand-binding abilities with various endogenous ligands present in the male and female reproductive tracts and may play a role in the reproduction process. Porcine spermadhesins (AQN, AWN, PSP protein families) are secreted mainly by the seminal vesicles, but their mRNAs have been found also in the cauda epididymis and prostate. Unlike AQN and AWN spermadhesins, localization of porcine seminal plasma (PSP) proteins in the boar reproductive tract has not been completely resolved. This work has focused on PSP protein expression and localization in the boar reproductive organs and on spermatozoa. Using specific rabbit polyclonal antibodies (anti-PSP I and anti-PSP II), PSP I and PSP II proteins were immunodetected in tissue extracts and in secretory tissues of cauda epididymis, prostate, seminal vesicles and Cowper's glands on the blots and by an indirect immunofluorescence technique, respectively. Moreover, the ability of PSP proteins to bind to epididymal spermatozoa indicated their presence on cauda epididymal and ejaculated spermatozoa. Porcine seminal plasma proteins bind to the sperm surface at ejaculation and may modulate several aspects of sperm activity during reproduction. PSP proteins are produced not only by seminal vesicles and prostate, but also by epididymis. However, their prospective role in sperm epididymal maturation is not clear. Further characterization of seminal plasma protein forms expressed in the individual reproductive organs will help to understand their subsequent role in the reproduction process.
精子黏附素是一类含有特征性CUB结构域的蛋白质，最初是从家畜的精浆和射出的精子中分离出来的。公猪精子黏附素是多功能蛋白质，对雄性和雌性生殖道中存在的各种内源性配体具有配体结合能力，可能在生殖过程中发挥作用。猪精子黏附素（AQN、AWN、PSP蛋白家族）主要由精囊分泌，但其mRNA也在附睾尾部和前列腺中被发现。与AQN和AWN精子黏附素不同，猪精浆（PSP）蛋白在公猪生殖道中的定位尚未完全明确。这项工作聚焦于PSP蛋白在公猪生殖器官和精子中的表达及定位。使用特异性兔多克隆抗体（抗-PSP I和抗-PSP II），分别通过印迹法在组织提取物以及附睾尾部、前列腺、精囊和尿道球腺的分泌组织中免疫检测到了PSP I和PSP II蛋白，并通过间接免疫荧光技术进行了检测。此外，PSP蛋白与附睾精子结合的能力表明它们存在于附睾尾部和射出的精子上。猪精浆蛋白在射精时与精子表面结合，并可能在生殖过程中调节精子活动的多个方面。PSP蛋白不仅由精囊和前列腺产生，附睾也能产生。然而，它们在精子附睾成熟过程中的潜在作用尚不清楚。对各个生殖器官中表达的精浆蛋白形式进行进一步表征，将有助于了解它们在生殖过程中的后续作用。
