Liberda Jirí, Manásková Pavla, Prelovská Lucie, Tichá Marie, Jonáková Vera
Department of Biochemistry, Charles University, Prague, Czech Republic.
J Reprod Immunol. 2006 Oct;71(2):112-25. doi: 10.1016/j.jri.2006.01.008. Epub 2006 Apr 5.
The role of boar seminal plasma proteins attached to the sperm plasma membrane during ejaculation has been studied in saccharide-mediated events in the female reproductive tract. Heparin-binding (Hep(+)) proteins (DQH sperm surface protein, and AQN and AWN spermadhesins) and their aggregated forms (fractions II and III) interacted more strongly with both oviductal epithelium cells and fluid than non-heparin-binding (Hep(-)) proteins (PSP I and PSP II spermadhesins) and their heterodimer (fraction IV), and interactions correlate with affinity of these proteins to yeast mannan. Indirect immunofluorescence (IMF) showed that the AQN 1 spermadhesin and fraction II bind to the apical glycocalyx of the ampulla, as well as the isthmic and uterine tubal junction regions of the oviductal sections. IMF demonstrated the recognition of AQN 1 and fraction II and mannosyl components of oviductal epithelium. We suggest that Hep(+) proteins (especially AQN 1, fraction II) on sperm could enable sperm binding to oviductal epithelium and thus participate in formation of the sperm oviductal reservoir. Interactions of Hep(+) proteins to oviductal epithelium were inhibited by mannan, hyaluronic acid and sialylated O-glycoproteins. No or slight inhibition was observed with sulphated polysaccharides (heparin, chondroitin sulphate) and simple monosaccharides. Besides that, attachment of boar seminal plasma proteins to oviductal epithelium cells was affected by oviductal fluid, the natural environment in the oviduct. Moreover, the ability of hyaluronic acid to inhibit the interaction of sperm surface proteins to the oviduct might play a role in sperm release from the oviductal reservoir and in the capacitation process.
射精时附着于精子质膜上的公猪精浆蛋白在雌性生殖道糖类介导的事件中的作用已得到研究。肝素结合（Hep(+)）蛋白（DQH精子表面蛋白、AQN和AWN精子黏附素）及其聚集形式（组分II和III）与输卵管上皮细胞和液体的相互作用比非肝素结合（Hep(-)）蛋白（PSP I和PSP II精子黏附素）及其异二聚体（组分IV）更强，且这些相互作用与这些蛋白对酵母甘露聚糖的亲和力相关。间接免疫荧光（IMF）显示，AQN 1精子黏附素和组分II与壶腹的顶端糖萼以及输卵管切片的峡部和子宫输卵管连接区域结合。IMF证明了输卵管上皮对AQN 1、组分II和甘露糖基成分的识别。我们认为，精子上的Hep(+)蛋白（尤其是AQN 1、组分II）可使精子与输卵管上皮结合，从而参与精子输卵管储存库的形成。Hep(+)蛋白与输卵管上皮的相互作用受到甘露聚糖、透明质酸和唾液酸化O-糖蛋白的抑制。硫酸化多糖（肝素、硫酸软骨素）和简单单糖未观察到抑制作用或仅有轻微抑制作用。除此之外，公猪精浆蛋白与输卵管上皮细胞的附着受到输卵管液（输卵管内的自然环境）的影响。此外，透明质酸抑制精子表面蛋白与输卵管相互作用的能力可能在精子从输卵管储存库释放及获能过程中发挥作用。
