Takahashi Syun, Yamazaki Tsutomu, Satoh Kozue, Inoue Miyuki, Takahashi Sachiko, Ishihara Osamu, Oka Yohko, Horiguchi Yuji, Okuwaki Yoshiyuki, Suzuki Satowa, Kishimoto Toshio
Department of Nutrition, Women's Collenge for Nutrition, Saitama 350-0288, Japan.
Jpn J Infect Dis. 2007 Nov;60(6):374-6.
The objective of this study was to clarify the longitudinal epidemiology of Chlamydia trachomatis serovars in Japan. A total of 339 endocervical swab specimens obtained from female patients who attended the Department of Obstetrics and Gynecology, Saitama Medical School, were used. Positive specimens of either transport medium of IDEIA Chlamydia (1st group, from 1999 to 2001), or DNA extract of Cobas Amplicor STD-1 Chlamydia trachomatis (2nd group, from 2003 to 2005) were used for serotyping. Typing of C. trachomatis serovars in DNA extracts was performed by polymerase chain reaction-restriction fragment length polymorphism. Ten serovars, A, B, D, E, F, G, H, I, J and K, were identified in the 1st group, and serovar E was most frequently identified (27.6%). In the 2nd group, nine serovars, B, C, D, E, F, G, H, I and K, were identified, and serovar D was most frequently identified (24.7%). Serovars B and Ba were significantly more common around 2000 and the mid-1990s (from 1993 to 1996), respectively. Numbers of serovar I increased significantly during the research period. In addition, serovar I was more frequent in the 2nd group than in the 1st group in women aged 20-29 years. There were no significant differences of serovar distribution between pregnant and non-pregnant women.
本研究的目的是阐明日本沙眼衣原体血清型的纵向流行病学情况。共使用了从埼玉医科大学妇产科就诊的女性患者中获取的339份宫颈拭子标本。对IDEIA衣原体运输培养基阳性标本（第一组，1999年至2001年）或Cobas Amplicor STD - 1沙眼衣原体DNA提取物（第二组，2003年至2005年）进行血清分型。通过聚合酶链反应 - 限制性片段长度多态性对DNA提取物中的沙眼衣原体血清型进行分型。在第一组中鉴定出10种血清型，即A、B、D、E、F、G、H、I、J和K，其中血清型E最常见（27.6%）。在第二组中鉴定出9种血清型，即B、C、D、E、F、G、H、I和K，其中血清型D最常见（24.7%）。血清型B和Ba分别在2000年左右和20世纪90年代中期（1993年至1996年）明显更为常见。在研究期间，血清型I的数量显著增加。此外，在20至29岁的女性中，血清型I在第二组中比在第一组中更常见。孕妇和非孕妇之间血清型分布没有显著差异。
