Transport kinetics of glucose and alanine in renal brush-border membrane vesicles of cadmium-intoxicated rabbits.

Changes in transport kinetics of D-glucose and L-alanine were studied in renal luminal membrane vesicles isolated from cadmium-intoxicated rabbits. Cadmium intoxication was induced by subcutaneous injections of CdCl2 at a dose of 2 mg Cd/kg/day for 2-3 weeks. Brush-border membrane vesicles were prepared from renal outer cortex and outer medulla by Percoll gradient centrifugation. Cadmium intoxication resulted in a marked attenuation of Na(+)-dependent transports of D-glucose and L-alanine in both outer cortical and outer medullary brush-border membrane vesicles, and this was due to reduction in Vmax and not Km. Similar results were obtained in normal vesicles directly exposed to free cadmium. These results suggest that in long-term cadmium-exposed animals free cadmium ions liberated in the proximal tubular cytoplasm may directly impair brush-border membranes thereby reducing capacity of Na(+)-dependent transport systems for glucose and amino acids.