Basabe-Desmonts Lourdes, Benito-López Fernando, Gardeniers Han J G E, Duwel Rob, van den Berg Albert, Reinhoudt David N, Crego-Calama Mercedes
Department of Supramolecular Chemistry and Technology, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.
Anal Bioanal Chem. 2008 Jan;390(1):307-15. doi: 10.1007/s00216-007-1720-2. Epub 2007 Nov 23.
Miniaturization and automation are highly important issues for the development of high-throughput processes. The area of micro total analysis systems (muTAS) is growing rapidly and the design of new schemes which are suitable for miniaturized analytical devices is of great importance. In this paper we report the immobilization of self-assembled monolayers (SAMs) with metal ion sensing properties, on the walls of glass microchannels. The parallel combinatorial synthesis of sensing SAMs in individually addressable microchannels towards the generation of optical sensor arrays and sensing chips has been developed. [figure: see text] The advantages of microfluidic devices, surface chemistry, parallel synthesis, and combinatorial approaches have been merged to integrate a fluorescent chemical sensor array in a microfluidic chip. Specifically, five different fluorescent self-assembled monolayers have been created on the internal walls of glass microchannels confined in a microfluidic chip.
小型化和自动化是高通量过程发展的极为重要的问题。微全分析系统（μTAS）领域正在迅速发展，设计适用于小型化分析设备的新方案至关重要。在本文中，我们报道了将具有金属离子传感特性的自组装单分子层（SAMs）固定在玻璃微通道壁上。已开发出在可单独寻址的微通道中对传感SAMs进行平行组合合成，以生成光学传感器阵列和传感芯片。[图：见正文]微流体装置、表面化学、平行合成和组合方法的优势已被融合，以在微流体芯片中集成荧光化学传感器阵列。具体而言，在微流体芯片内的玻璃微通道内壁上创建了五种不同的荧光自组装单分子层。
