Sevillano Xabier, Isasi José R, Peñas Francisco J
Department of Chemistry and Soil Science, University of Navarra, Pamplona, Spain.
Biodegradation. 2008 Jul;19(4):589-97. doi: 10.1007/s10532-007-9164-0. Epub 2007 Nov 23.
This work is focused on the evaluation of a beta-cyclodextrin polymer as a carrier medium in a fluidized bed bioreactor treating aqueous phenol as a model pollutant. The insoluble polymer support was obtained in the shape of spherical beads by crosslinking beta-cyclodextrin with epichlorohydrin. A batch of swollen polymer particles was loaded into the reactor and inoculated with a mixed bacterial culture. Bacterial growth on the polymer beads was initially stimulated by glucose addition to the medium, and then gradually replaced with phenol. The operational variables studied after the acclimation period included phenol load, hydraulic residence time and recirculation flow rate. Low hydraulic residence times and moderate phenol loads were applied. The elimination capacity was usually about 1.0 kg-phenol/m(3)d, although a maximum of 2.8 kg-phenol/m(3)d was achieved with a retention time of only 0.55 h. The depuration efficiency was not affected by the recirculation flow rate in the range studied. Neither operational nor support stability problems were detected during the operation. A high degree of expansion was achieved in the bioreactor due to the hydrogel nature of the cyclodextrin polymer and, consequently, a low energy requirement was necessary to fluidize the bed.
本研究聚焦于评估β-环糊精聚合物作为流化床生物反应器中的载体介质，处理作为模型污染物的苯酚水溶液的性能。通过β-环糊精与环氧氯丙烷交联，获得了球形珠状的不溶性聚合物载体。将一批溶胀的聚合物颗粒装入反应器，并接种混合细菌培养物。聚合物珠粒上的细菌生长最初通过向培养基中添加葡萄糖来刺激，然后逐渐用苯酚替代。驯化期后研究的操作变量包括苯酚负荷、水力停留时间和循环流速。采用了较短的水力停留时间和适中的苯酚负荷。尽管在仅0.55小时的停留时间下达到了最高2.8 kg-苯酚/m³·d的去除能力，但去除能力通常约为1.0 kg-苯酚/m³·d。在所研究的范围内，净化效率不受循环流速的影响。运行过程中未检测到操作稳定性和载体稳定性问题。由于环糊精聚合物的水凝胶性质，生物反应器实现了高度膨胀，因此流化床所需的能量较低。
