[巴西固氮螺菌Sp7细胞与多克隆抗体相互作用过程中电物理特性的变化]

[Changes in electrophysical characteristics of Azospirillum brasilense Sp7 cells during their interaction with polyclonal antibodies].

作者信息

Guliĭ O I, Bunin V D, O'Neĭl D, Ivnitskiĭ D, Matora L Iu, Ignatov V V, Ignatov O V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2007 Sep-Oct(5):80-2.

Abstract

Electrooptical characteristics of Azospirillum brasilense Sp7 cells during their specific interaction with polyclonal rabbit antibodies were studied. Dependence of optical density of cell suspension during electroorientation of cells from frequency of orienting field in interval 10, 100, 250, and 500 kHz was evaluated. Itwas shown that electrooptical (EO) characteristics of bacterial suspensions change during interaction of A. brasilense cells with antibodies, and maximal changes occur when frequency of oriented field amounts 100-250 kHz. During interaction of A. brasilense Sp7 with strain-specific polyclonal antibodies in the presence of Escherichia coli K-12 and Pseudomonas putida C-11 decrease of amplitude of analytic signal was observed but detection of A. brasilense Sp7 cells was possible. Possibility of detection of microorganisms by EO analysis during their interaction with antibodies was shown.

摘要

研究了巴西固氮螺菌Sp7细胞与多克隆兔抗体特异性相互作用过程中的电光特性。评估了细胞悬液在10、100、250和500kHz频率的定向场作用下细胞电取向过程中光密度与定向场频率的关系。结果表明，在巴西固氮螺菌细胞与抗体相互作用过程中，细菌悬液的电光（EO）特性发生变化，当定向场频率为100 - 250kHz时变化最大。在大肠杆菌K - 12和恶臭假单胞菌C - 11存在的情况下，巴西固氮螺菌Sp7与菌株特异性多克隆抗体相互作用时，分析信号幅度降低，但仍可检测到巴西固氮螺菌Sp7细胞。研究表明，在微生物与抗体相互作用过程中，通过电光分析检测微生物是可行的。