Belovolova L V, Bliumenfel'd L A, Burbaev D Sh, Vanin A F
Mol Biol (Mosk). 1975 Nov-Dec;9(6):934-40.
The conditions of structural modifications of horse heart cytochrome c (pH, salt concentration) have been studied. Under these conditions the rate of carboxycytochrome c formation greatly increases in the course of the reduction process as compared to this rate after cytochrome c reduction and relaxation to the equilibrium state. According to these results the reduced intermediate which appears in the course of reduction has a high affinity for the carbon monoxide. It has been shown that the reduced low-spin cytochrome c practically does not take part in the process of dynamic conformational equilibrium with other cytochrome c forms existing in equilibrium mixture of oxidized and reduced cytochrome c.
对马心细胞色素c结构修饰的条件（pH值、盐浓度）进行了研究。在这些条件下，与细胞色素c还原并松弛至平衡状态后的速率相比，在还原过程中羧基细胞色素c的形成速率大幅增加。根据这些结果，还原过程中出现的还原中间体对一氧化碳具有高亲和力。研究表明，还原态的低自旋细胞色素c实际上不参与与氧化态和还原态细胞色素c平衡混合物中存在的其他细胞色素c形式的动态构象平衡过程。
