Grzelak I, Olszewski W L, Rowiński W
Laboratory of Surgical Research and Transplantation, Polish Academy of Sciences, Warsaw.
Arch Immunol Ther Exp (Warsz). 1991;39(1-2):133-8.
The influence of moderate operative trauma on lymphocyte responsiveness to phytohemagglutin (PHA) and in autologous mixed lymphocyte response (AMLR) was studied. Furthermore, production of interleukin 2 (IL2) and immunomodulatory effect of exogenous IL2 on lymphocyte reactivity after surgery were examined. The responsiveness to PHA declined on day 1 (p less than 0.05) and the level of AMLR was found decreased on days 1 (p less than 0.05), 3 (p less than 0.02) and 5 (p less than 0.03) after surgery. Production of IL2 significantly decreased on day 1, 3 and 5 (p less than 0.05) after operation. Supplementation of PHA and AMLR cultures with exogenous IL2 restored the attenuated postoperative responsiveness to the levels observed preoperatively. This observation may justify IL2 therapy in surgical patients with high risk of uncontrollable postoperative infections.
研究了中度手术创伤对淋巴细胞对植物血凝素（PHA）反应性及自体混合淋巴细胞反应（AMLR）的影响。此外，还检测了白细胞介素2（IL2）的产生以及外源性IL2对术后淋巴细胞反应性的免疫调节作用。术后第1天对PHA的反应性下降（p<0.05），术后第1天（p<0.05）、第3天（p<0.02）和第5天（p<0.03）发现AMLR水平降低。术后第1天、第3天和第5天IL2的产生显著减少（p<0.05）。用外源性IL2补充PHA和AMLR培养物可将术后减弱的反应性恢复到术前观察到的水平。这一观察结果可能为术后有不可控感染高风险的手术患者进行IL2治疗提供依据。
