Li Liyuan, Wada Masaru, Yokota Atsushi
Laboratory of Microbial Physiology, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan.
Proteomics. 2007 Dec;7(23):4317-22. doi: 10.1002/pmic.200700269.
We constructed a cytoplasmic proteome reference map for a glutamic acid producing Corynebacterium glutamicum ATCC 14067 by 2-DE and protein identification by MALDI-TOF-MS and PMF using genome database of the type strain ATCC 13032. The map allowed us to identify 166 protein spots representing 139 different proteins. A considerable strain difference was observed in the proteomic images between strains ATCC 14067 and ATCC 13032 grown under the glutamic acid production conditions, suggesting the importance of strain-specific reference map for proteomic analysis.
我们通过二维电泳（2-DE）以及利用模式菌株ATCC 13032的基因组数据库进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和肽质量指纹图谱（PMF）蛋白质鉴定，构建了谷氨酸生产菌谷氨酸棒杆菌ATCC 14067的细胞质蛋白质组参考图谱。该图谱使我们能够鉴定出代表139种不同蛋白质的166个蛋白质点。在谷氨酸生产条件下生长的ATCC 14067和ATCC 13032菌株之间的蛋白质组图像中观察到了显著的菌株差异，这表明菌株特异性参考图谱在蛋白质组分析中的重要性。
