谷氨酸生产菌谷氨酸棒杆菌ATCC 14067的细胞质蛋白质组参考图谱。

Cytoplasmic proteome reference map for a glutamic acid-producing Corynebacterium glutamicum ATCC 14067.

作者信息

Li Liyuan, Wada Masaru, Yokota Atsushi

机构信息

Laboratory of Microbial Physiology, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan.

出版信息

Proteomics. 2007 Dec;7(23):4317-22. doi: 10.1002/pmic.200700269.

DOI:10.1002/pmic.200700269

PMID:18040983

Abstract

We constructed a cytoplasmic proteome reference map for a glutamic acid producing Corynebacterium glutamicum ATCC 14067 by 2-DE and protein identification by MALDI-TOF-MS and PMF using genome database of the type strain ATCC 13032. The map allowed us to identify 166 protein spots representing 139 different proteins. A considerable strain difference was observed in the proteomic images between strains ATCC 14067 and ATCC 13032 grown under the glutamic acid production conditions, suggesting the importance of strain-specific reference map for proteomic analysis.

摘要

我们通过二维电泳（2-DE）以及利用模式菌株ATCC 13032的基因组数据库进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和肽质量指纹图谱（PMF）蛋白质鉴定，构建了谷氨酸生产菌谷氨酸棒杆菌ATCC 14067的细胞质蛋白质组参考图谱。该图谱使我们能够鉴定出代表139种不同蛋白质的166个蛋白质点。在谷氨酸生产条件下生长的ATCC 14067和ATCC 13032菌株之间的蛋白质组图像中观察到了显著的菌株差异，这表明菌株特异性参考图谱在蛋白质组分析中的重要性。