Soltanian S, Dhont J, Sorgeloos P, Bossier P
Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University, Belgium.
J Appl Microbiol. 2008 Apr;104(4):1137-46. doi: 10.1111/j.1365-2672.2007.03629.x. Epub 2007 Nov 27.
To evaluate nutritional and anti-infectious characteristics of the chemically treated baker's yeast with 2-mercapto-ethanol (2ME) for gnotobiotically grown Artemia.
A selection of isogenic yeast strains was treated with 2ME and fed to gnotobiotically grown Artemia. In the first experiment the effect of the chemical treatment on the yeast nutritional value was studied. In most cases, 2ME-treated yeast cells were better feed for Artemia than the untreated cells. In the second experiment, a small quantity of 2ME-treated yeast cells was fed to Vibrio campbellii (VC) challenged Artemia. The 2ME-treatment on some yeast strains (e.g. gas1, kre6 and chs3) significantly improved Artemia resistance against VC compared with the respective untreated yeast cells.
Simple chemical treatment with 2ME could significantly improve the nutritional and anti-infectious properties of some baker's yeast strains for gnotobiotically grown Artemia.
The gnotobiotic Artemia test system provides a unique opportunity (because of noninterference of other microbial compounds) to investigate how the yeast cell wall composition influences macro parameters (e.g. growth and survival) in an organism. In addition, gene expression studies in these gnotobiotically grown Artemia should provide further documentation on direct effects of yeast cells on the genes involved in immune functions.
评估经2-巯基乙醇（2ME）化学处理的面包酵母对无菌培养的卤虫的营养及抗感染特性。
选用同基因酵母菌株，用2ME处理后投喂给无菌培养的卤虫。在第一个实验中，研究了化学处理对酵母营养价值的影响。在大多数情况下，经2ME处理的酵母细胞比未处理的细胞更适合作为卤虫的饲料。在第二个实验中，将少量经2ME处理的酵母细胞投喂给受坎氏弧菌（VC）攻击的卤虫。与各自未处理的酵母细胞相比，对某些酵母菌株（如gas1、kre6和chs3）进行2ME处理可显著提高卤虫对VC的抵抗力。
用2ME进行简单的化学处理可显著提高某些面包酵母菌株对无菌培养卤虫的营养和抗感染特性。
无菌卤虫测试系统提供了一个独特的机会（由于不受其他微生物化合物的干扰）来研究酵母细胞壁组成如何影响生物体中的宏观参数（如生长和存活）。此外，对这些无菌培养卤虫的基因表达研究应能进一步证明酵母细胞对免疫功能相关基因的直接影响。
