Shin M H, Ryu J S, Min D Y
Department of Parasitology, College of Medicine, Hanyang University, Seoul, Korea.
Kisaengchunghak Chapchi. 1991 Dec;29(4):397-401. doi: 10.3347/kjp.1991.29.4.397.
Paragonimus westermani is a common fluke in Korea. The present study aimed to determine serum total IgE and specific IgG levels in experimental paragonimiasis of rats. Each Wistar rat was inoculated orally with 20-25 metacercariae of P. westermani from Cambaroides similis. Before and after infection (1, 2, 3, 4, 6, 8 weeks) of P. westermani, the blood was collected from the retro-orbital venous plexus of rats and kept serum at -70 degrees C. Serum total IgE and specific IgG levels were determined by the capture and conventional enzyme-linked immunosorbent assay, respectively. The results were as follows; 1. Serum IgE values were increased to 0.18 +/- 0.042 at 2 weeks, 0.28 +/- 0.151 at 4 weeks and 0.43 +/- 0.055 at 8 weeks after infection. The absorbances of non-infected rats ranged 0.07 +/- 0.021-0.12 +/- 0.025. 2. Specific IgG values were slightly increased at 3 weeks (0.20 +/- 0.032) and gradually increased up to 8 weeks (0.31 +/- 0.067) after infection. The absorbances of non-infected rats ranged 0.11 +/- 0.035-0.18 +/- 0.019. The present results suggested that P. westermani could elevate serum IgE and specific IgG antibodies in Wistar rats which were not a good definitive host.
卫氏并殖吸虫是韩国一种常见的吸虫。本研究旨在测定大鼠实验性并殖吸虫病血清总IgE和特异性IgG水平。每只Wistar大鼠经口接种来自东北蝲蛄的20 - 25条卫氏并殖吸虫囊蚴。在感染卫氏并殖吸虫之前及之后（1、2、3、4、6、8周），从大鼠眼眶后静脉丛采集血液，并将血清保存在-70℃。血清总IgE和特异性IgG水平分别通过捕获法和常规酶联免疫吸附测定法测定。结果如下：1. 感染后2周血清IgE值增至0.18±0.042，4周时为0.28±0.151，8周时为0.43±0.055。未感染大鼠的吸光度范围为0.07±0.021 - 0.12±0.025。2. 特异性IgG值在感染后3周略有增加（0.20±0.032），并在8周时逐渐增至0.31±0.067。未感染大鼠的吸光度范围为0.11±0.035 - 0.18±0.019。目前的结果表明，卫氏并殖吸虫可使Wistar大鼠（并非适宜的终宿主）血清IgE和特异性IgG抗体升高。
