Canteros B, Minsavage G, Bonas U, Pring D, Stall R
Department of Plant Pathology, University of Florida, Gainesville.
Mol Plant Microbe Interact. 1991 Nov-Dec;4(6):628-32. doi: 10.1094/mpmi-4-628.
Strains of Xanthomonas campestris pv. vesicatoria that were avirulent in tomato leaves but virulent in pepper leaves were identified. A cloned gene, avrBsP, from one of the strains, Xv 87-7, converted a virulent strain in tomato to avirulent in tomato. A 1.7-kb subclone containing the avirulence gene cross-hybridized with the avirulence gene, which determines race 1 within the pepper group of strains (avrBs3). However, the two avirulence genes differ in their biological activity. The base sequences of the two avirulence genes were almost identical through the 1.7-kb segment of avrBsP, with significant differences only in some bases in the repeat region.
已鉴定出在番茄叶片中无毒性但在辣椒叶片中有毒性的野油菜黄单胞菌斑点病致病变种菌株。从其中一个菌株Xv 87 - 7克隆得到的基因avrBsP，可使番茄中的一个毒性菌株转变为无毒性菌株。一个包含无毒基因的1.7 kb亚克隆与无毒基因发生交叉杂交，该无毒基因决定了辣椒菌株组内的1号生理小种（avrBs3）。然而，这两个无毒基因在生物学活性上有所不同。两个无毒基因的碱基序列在avrBsP的1.7 kb片段上几乎相同，仅在重复区域的某些碱基上存在显著差异。
