[Polymorphism Lys751Gln of XPD gene and breast cancer risk in women].

UNLABELLED

Polymorphisms in DNA repair genes resulting in variation of DNA repair efficiency may therefore be associated with cancer risk.

AIM OF THE STUDY

In the present work the distribution of genotypes and frequency of alleles of the Lys751Gln polymorphism of XPD gene in subjects with breast cancer were investigated.

MATERIAL AND METHODS

Genomic DNA isolated from 92 breast cancer patients and control (n=110) was used to genotype XPD Lys751Gln by means of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis.

RESULTS

The distribution of the genotypes of the Lys751Gln polymorphism of XPD in both control and patients did not differ significantly (p > 0.05) from those predicted by the Hardy-Weinberg distribution. There were no significant differences (p > 0.05) in genotype distributions and allele frequencies between subgroups assigned to histological stage.

CONCLUSIONS

The results suggest that the Lys751Gln polymorphism of XPD gene may not be linked with appearance and development of breast cancer.