通过高压液相色谱法对生长调节木葡聚糖衍生寡糖进行纯化与分析。

Purification and analysis of growth-regulating xyloglucan-derived oligosaccharides by high-pressure liquid chromatography.

作者信息

McDougall G J, Fry S C

机构信息

Department of Botany, University of Edinburgh, Great Britain.

出版信息

Carbohydr Res. 1991 Oct 14;219:123-32. doi: 10.1016/0008-6215(91)89047-j.

DOI:10.1016/0008-6215(91)89047-j

PMID:1804529

Abstract

The retention times of 10 oligosaccharides, generated from the xyloglucans of Rosa and Tropaeolum by the action of Trichoderma cellulase, and of 17 related carbohydrates, in h.p.l.c. on an amino-substituted silica (Amino-Spheri-5) depended largely on the number of hydroxyl groups per molecule, whereas h.p.l.c. on a pellicular anion-exchange resin (CarboPac PA1) was strongly influenced by the nature of the sugar residues present, especially L-fucose, and by their linkages. The major nonasaccharide (XG9, D-Glc4-D-Xyl3-D-Gal-L-Fuc) obtained from Rosa xyloglucan, after purification on Amino-Spheri-5, retained biological activity as an inhibitor of auxin-induced growth in a Pisum stem-segment bioassay. H.p.l.c. on Amino-Spheri-5 was used to monitor the action of "Driselase" in stripping the non-reducing terminal alpha-D-Xylp-(1----6)-beta-D-Glcp units from XG9 to yield a pentasaccharide (XG5, D-Glc2-D-Xyl-D-Gal-L-Fuc).

摘要

由木霉纤维素酶作用于蔷薇属和旱金莲属的木葡聚糖所产生的10种低聚糖以及17种相关碳水化合物在氨基取代硅胶（Amino-Spheri-5）上进行高效液相色谱分析时，其保留时间在很大程度上取决于每个分子中羟基的数量，而在薄壳阴离子交换树脂（CarboPac PA1）上进行高效液相色谱分析时，则受到存在的糖残基的性质（尤其是L-岩藻糖）及其连接方式的强烈影响。从蔷薇属木葡聚糖中获得的主要九糖（XG9，D-Glc4-D-Xyl3-D-Gal-L-Fuc）在Amino-Spheri-5上纯化后，在豌豆茎段生物测定中作为生长素诱导生长的抑制剂保留了生物活性。利用Amino-Spheri-5进行高效液相色谱分析来监测“Driselase”从XG9上剥离非还原末端α-D-Xylp-(1→6)-β-D-Glcp单元以产生五糖（XG5，D-Glc2-D-Xyl-D-Gal-L-Fuc）的作用。