Hantus S, Pauly M, Darvill A G, Albersheim P, York W S
Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712, USA.
Carbohydr Res. 1997 Oct 28;304(1):11-20. doi: 10.1016/s0008-6215(97)00200-0.
Jojoba seed xyloglucan was shown to be a convenient source of biologically active xyloglucan oligosaccharides that contain both L- and D-galactosyl residues [E. Zablackis et al., Science, 272 (1996) 1808-1810]. Oligosaccharides were isolated by liquid chromatography of the mixture of oligosaccharides generated by treating jojoba seed xyloglucan with a beta-(1-->4)-endoglucanase. The purified oligosaccharides were reduced with NaBH4, converting them to oligoglycosyl alditol derivatives that were structurally characterized by a combination of mass spectrometry and 2-dimensional NMR spectroscopy. This analysis established that jojoba xyloglucan oligosaccharides contain the novel side-chain [alpha-L-Gal p-(1-->2)-beta-D-Galp-(1-->2)-alpha-D-Xyl p-(1-->6)-], which is structurally homologous to the fucose-containing side-chain [alpha-L-Fucp-(1-->2)-beta-D-Galp-(1-->2)-alpha-D-Xyl p-(1-->6)-] found in other biologically active xyloglucan oligosaccharides.
霍霍巴籽木葡聚糖被证明是生物活性木葡寡糖的便捷来源，这些寡糖同时含有L-和D-半乳糖基残基[E. 扎布拉斯基等人，《科学》，272 (1996) 1808 - 1810]。通过用β-(1→4)-内切葡聚糖酶处理霍霍巴籽木葡聚糖产生的寡糖混合物进行液相色谱分离寡糖。纯化后的寡糖用NaBH4还原，将它们转化为寡糖基糖醇衍生物，通过质谱和二维核磁共振光谱相结合对其结构进行表征。该分析确定霍霍巴木葡寡糖含有新型侧链[α-L-Gal p-(1→2)-β-D-Galp-(1→2)-α-D-Xyl p-(1→6)-]，其结构与其他生物活性木葡寡糖中发现的含岩藻糖侧链[α-L-Fucp-(1→2)-β-D-Galp-(1→2)-α-D-Xyl p-(1→6)-]同源。
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