Elshibli Sakina, Korpelainen Helena
Department of Applied Biology, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland.
Genetica. 2008 Oct;134(2):251-60. doi: 10.1007/s10709-007-9232-8. Epub 2007 Nov 29.
Genetic diversity in date palm germplasm from Sudan representing 37 female and 23 male accessions was investigated using 16 loci of microsatellite (SSR) primers. Eight female accessions from Morocco were included as reference material. The tested SSR markers showed a high level of polymorphism. A total of 343 alleles were detected at the 16 loci. The number of alleles per marker ranged from 14 to 44 with an average of 21.4 per locus. A high level of expected heterozygosity was observed among Sudan cultivars (0.841), Morocco cultivars (0.820) and male accessions (0.799). The results indicate that the genetic groups of the Sudan cultivars and/or males do not follow a clear geographic pattern. However, the morocco group showed significant differentiation in relation to the Sudan groups, as measured by F (ST) values and genetic distances. The effect of the methods of pollination and cultivar selection on the genetic structure was clearly detected by the weak clustering association that was observed for the majority of accessions originating from Sudan and Morocco as well. This suggests the need for further investigation on the genetic diversity of Sudanese date palm germplasm. A deeper insight will be revealed by a detailed analysis of populations originating from different geographic locations.
利用16个微卫星（SSR）引物位点，对来自苏丹的37份雌性和23份雄性海枣种质资源的遗传多样性进行了研究。其中包括8份来自摩洛哥的雌性种质作为参考材料。所测试的SSR标记显示出高度的多态性。在16个位点共检测到343个等位基因。每个标记的等位基因数量从14到44不等，平均每个位点为21.4个。在苏丹品种（0.841）、摩洛哥品种（0.820）和雄性种质（0.799）中观察到较高水平的预期杂合度。结果表明，苏丹品种和/或雄性的遗传群体并不遵循明确的地理模式。然而，通过F（ST）值和遗传距离测量，摩洛哥群体与苏丹群体相比显示出显著差异。授粉方法和品种选择对遗传结构的影响通过对大多数来自苏丹和摩洛哥的种质观察到的弱聚类关联清晰地检测到。这表明需要对苏丹海枣种质资源的遗传多样性进行进一步研究。对来自不同地理位置的群体进行详细分析将揭示更深入的见解。
