Ohno M, Yamamoto T, Watanabe S
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Eur J Pharmacol. 1991 Oct 29;204(1):113-6. doi: 10.1016/0014-2999(91)90844-g.
A 5-min period of cerebral ischemia induced in rats by the four-vessel occlusion method increased the number of errors (attempts to pass through two incorrect panels of the three panel-gates at four choice points) assessed by a working memory procedure applied in a three-panel runway task. The protein kinase C (PKC) inhibitor, staurosporine 0.03 and 0.1 mg/kg, administered immediately after blood flow reperfusion, significantly reduced the increase in errors expected to occur 24 h after 5 min of ischemia. However, administration of staurosporine 0.1 mg/kg 6 h after ischemia had no effect on the increase in errors. The protective effect of staurosporine suggests that the enhanced PKC activity during the early reperfusion phase plays a crucial role in the post-ischemic impairment of working memory.
采用四动脉闭塞法在大鼠中诱导5分钟的脑缺血，通过在三板式跑道任务中应用的工作记忆程序评估，这增加了错误数量（在四个选择点尝试穿过三个板门中两个不正确的板）。蛋白激酶C（PKC）抑制剂，0.03和0.1mg/kg的星形孢菌素，在血流再灌注后立即给药，显著降低了预计在5分钟缺血后24小时出现的错误增加。然而，缺血6小时后给予0.1mg/kg的星形孢菌素对错误增加没有影响。星形孢菌素的保护作用表明，早期再灌注阶段PKC活性增强在缺血后工作记忆损害中起关键作用。
