Parinov Serguei, Emelyanov Alexander
Temasek Life Sciences Laboratory, Research Link, The National University of Singapore, 117604, Singapore.
Genome Biol. 2007;8 Suppl 1(Suppl 1):S6. doi: 10.1186/gb-2007-8-s1-s6.
The recent introduction of several transposable elements in zebrafish opens new frontiers for genetic manipulation in this important vertebrate model. This review discusses transposable elements as mutagenesis tools for fish functional genomics. We review various mutagenesis strategies that were previously applied in other genetic models, such as Drosophila, Arabidopsis, and mouse, that may be beneficial if applied in fish. We also discuss the forthcoming challenges of high-throughput functional genomics in fish.
斑马鱼中最近引入的几种转座元件为这个重要的脊椎动物模型中的基因操作开辟了新的前沿领域。本综述讨论了转座元件作为鱼类功能基因组学的诱变工具。我们回顾了以前在其他遗传模型(如果蝇、拟南芥和小鼠)中应用的各种诱变策略,这些策略如果应用于鱼类可能会有益处。我们还讨论了鱼类高通量功能基因组学即将面临的挑战。