Aleixo J A, Barbosa R C, Gil-Turnes C
Centro de Biotecnologia, Universidade Federal de Pelotas, UFPel, RS, Brazil.
Hybridoma. 1991 Oct;10(5):625-31. doi: 10.1089/hyb.1991.10.625.
Six hybridoma lines producing monoclonal antibodies (MAbs) against Moraxella bovis were established from fusions between the SP2/0 myeloma cells and BALB/c mice splenocytes. Three antibodies were of the IgG1 isotype, two were IgG2a, and one was IgG2b. The specificity of the antibodies was determined by indirect enzyme-linked immunosorbent assay (ELISA) using whole cells of M. bovis and of other Gram-negative bacteria, and lipopolysaccharide (LPS) from M. bovis JUR2 and E. coli as antigens. Ascitic fluid produced by the six hybridoma lines inhibited hemagglutination by M. bovis GF9. One MAb (35F) reacted specifically with purified M. bovis LPS in the ELISA test. The MAb panel detected heterogeneity among the isolates recovered from different geographical regions.
通过SP2/0骨髓瘤细胞与BALB/c小鼠脾细胞融合，建立了6株产生抗牛莫拉菌单克隆抗体（MAb）的杂交瘤细胞系。其中3种抗体为IgG1亚型，2种为IgG2a，1种为IgG2b。使用牛莫拉菌和其他革兰氏阴性菌的全细胞，以及牛莫拉菌JUR2和大肠杆菌的脂多糖（LPS）作为抗原，通过间接酶联免疫吸附测定（ELISA）确定抗体的特异性。6个杂交瘤细胞系产生的腹水可抑制牛莫拉菌GF9的血凝反应。在ELISA试验中，一种单克隆抗体（35F）与纯化的牛莫拉菌LPS发生特异性反应。该单克隆抗体组检测到从不同地理区域分离出的菌株之间存在异质性。
