Hong Byoung Seok, Kim Jeong Ho, Kim Na Yeon, Kim Bong-Gyu, Chong Youhoon, Ahn Joong-Hoon
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea.
J Biochem Mol Biol. 2007 Nov 30;40(6):870-4. doi: 10.5483/bmbrep.2007.40.6.870.
We cloned a uridine-diphosphate dependent glycosyltransferase RUGT-10 from Oryza sativa. The recombinant enzyme was expressed by glutathione-S transferase gene fusion system in Escherichia coli. RUGT10 showed different regioselectivity depending on the structures of substrates (e.g. flavanone, flavonol, and flavone). Apparently, flavanone such as naringenin and eriodictyol gave one 7-O-glucoside while flavone and flavonol gave more than two products with preferential glucosylation position of hydroxyl group at C-3 position.
我们从水稻中克隆了一种尿苷二磷酸依赖性糖基转移酶RUGT-10。该重组酶通过谷胱甘肽-S转移酶基因融合系统在大肠杆菌中表达。RUGT10根据底物（如黄烷酮、黄酮醇和黄酮）的结构表现出不同的区域选择性。显然，柚皮素和圣草酚等黄烷酮产生一种7-O-葡萄糖苷，而黄酮和黄酮醇产生两种以上产物，羟基优先在C-3位糖基化。
