Lee Ho Young, Jung Dae Young, Ha Hyekyung, Son Kun-Ho, Jeon Su-Jin, Kim Chungsook
Department of Drug Research & Development, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
J Biochem Mol Biol. 2007 Nov 30;40(6):1016-20. doi: 10.5483/bmbrep.2007.40.6.1016.
In this study, dioscin was isolated from Dioscoreae Rhizoma (DR), which is the rhizome of Dioscorea batatas D(ECNE). that inhabits broad areas of Korea and Japan. To determine whether dioscin induced growth hormone (GH) release, we evaluated its induction effects on GH release both in vitro and in vivo. The 70% methanol extract of DR, and its n-hexane and n-BuOH fractions, induced rat GH (rGH) release in rat pituitary cells 10-fold, 8-fold, and 5- fold higher than the control (0.36 +/- 0.02 nM), respectively (p < 0.05 each). The dioscin-induced rGH release of the cells was concentration-dependent and its ED(50) was 1.14 x 10(-5) M. Within 90 minutes after intravenous administration of 10 microg/kg (p < 0.05 at t(max)), dioscin caused the greatest increase in rGH concentration (C(max)) in the rat plasma (34.16 +/- 14.10 ng/ml) (n = 4), which was twice as high as the control group (12.88 +/- 3.29 ng/ml) (n = 27).
在本研究中，薯蓣皂苷从薯蓣根茎（DR）中分离得到，DR是薯蓣科植物薯蓣（Dioscorea batatas D(ECNE)）的根茎，广泛分布于韩国和日本。为确定薯蓣皂苷是否诱导生长激素（GH）释放，我们在体外和体内评估了其对GH释放的诱导作用。DR的70%甲醇提取物及其正己烷和正丁醇馏分，分别使大鼠垂体细胞中的大鼠生长激素（rGH）释放量比对照组（0.36±0.02 nM）高10倍、8倍和5倍（各p<0.05）。细胞中薯蓣皂苷诱导的rGH释放呈浓度依赖性，其半数有效剂量（ED(50)）为1.14×10(-5) M。静脉注射10μg/kg后90分钟内（t(max)时p<0.05），薯蓣皂苷使大鼠血浆中rGH浓度（C(max)）达到最大增幅（34.16±14.10 ng/ml）（n = 4），是对照组（12.88±3.29 ng/ml）（n = 27）的两倍。
