Sugiyama H, Gang W, Bergren V A, Eda R, Bergren D R, Hopp R J, Bewtra A K, Townley R G
Allergic Disease Center, Creighton University School of Medicine, Omaha, Nebr.
Int Arch Allergy Appl Immunol. 1991;96(3):206-10. doi: 10.1159/000235496.
We examined the effect of a new xanthine derivative, HWA448, on antigen-induced bronchoconstriction in actively sensitized guinea pigs. Guinea pigs were sensitized by intraperitoneal injection of bovine serum albumin (BSA) on two occasions, separated by 10 days. Two weeks after the second injection, the animal was placed in a two-chambered whole body plethysmograph and specific airway resistance (SRaw) was monitored for 10 min after an aerosol inhalation of BSA. HWA448 prevented the increase in SRaw after challenge (at 5 and 20 mg/kg i.p.). Aminophylline also prevented the increase in SRaw at 20 mg/kg, but not at a 5-mg/kg dose. The concentration of HWA448, which produced 50% relaxation of the tracheal rings constricted with 0.1 mM of histamine, was 49.9 microM as compared with 18.2 microM in aminophylline. HWA448 has a protective effect on antigen-induced bronchoconstriction in guinea pigs and may be a useful agent in the therapy of bronchial asthma.
我们研究了一种新型黄嘌呤衍生物HWA448对主动致敏豚鼠抗原诱导的支气管收缩的影响。豚鼠分两次腹腔注射牛血清白蛋白（BSA）进行致敏，两次注射间隔10天。第二次注射两周后，将动物置于两室全身体积描记器中，在雾化吸入BSA后监测10分钟的比气道阻力（SRaw）。HWA448可预防激发后SRaw的增加（腹腔注射5和20mg/kg时）。氨茶碱在20mg/kg时也可预防SRaw的增加，但在5mg/kg剂量时则不能。使被0.1mM组胺收缩的气管环产生50%舒张的HWA448浓度为49.9 microM，而氨茶碱为18.2 microM。HWA448对豚鼠抗原诱导的支气管收缩具有保护作用，可能是治疗支气管哮喘的有用药物。
