Müller R, Glathe H, Lang H, Simon H, Clausnitzer R, Petzold G, Dittmann S
Central Institute of Hygiene, Microbiology and Epidemiology, Berlin, F.R.G.
J Virol Methods. 1991 Sep-Oct;34(2):141-8. doi: 10.1016/0166-0934(91)90094-g.
A rapid and simple screening test for antibodies to HIV-1 was designed on the principle of dot-EIA. Recombinant HIV-1 env and gag polypeptides are fixed on nitrocellulose sheets. Peroxidase conjugated protein A is used for detection of bound antibodies. After addition of hydrogen peroxide and 2-bromo-1-naphtol antigen-antibody complexes are visualized as discrete blue coloured spots. The test is completed within 15 min. Out of 111 sera positive by commercial EIA and Western blot analysis 110 were recognized by dot-EIA (sensitivity: 99.1%). False positive results compared with commercial EIA were found in 2 of 423 healthy blood donors (specificity: 99.5%).
基于斑点酶免疫测定原理设计了一种快速简便的HIV-1抗体筛查试验。将重组HIV-1包膜蛋白和核衣壳蛋白多肽固定在硝酸纤维素膜上。用辣根过氧化物酶标记的蛋白A检测结合的抗体。加入过氧化氢和2-溴-1-萘酚后，抗原抗体复合物呈现为离散的蓝色斑点。该试验在15分钟内完成。在111份经商业酶免疫测定和蛋白质印迹分析呈阳性的血清中，110份被斑点酶免疫测定法识别（灵敏度：99.1%）。在423名健康献血者中，有2人出现与商业酶免疫测定法相比的假阳性结果（特异性：99.5%）。
