Peisajovich Sergio G, Tawfik Dan S
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel.
Nat Methods. 2007 Dec;4(12):991-4. doi: 10.1038/nmeth1207-991.
When generating novel tailor-made proteins, protein engineers routinely apply the principles of 'Darwinian' evolution. However, laboratory evolution of proteins also has the potential to test evolutionary theories and reproduce evolutionary scenarios, thus reconstructing putative protein intermediates and providing a glimpse of 'protein fossils'. This commentary describes research at the interface of applied and fundamental molecular evolution, and provides a personal view of how synergy between fundamental and applied experiments indicates novel and more efficient ways of generating new proteins in the laboratory.
在生成新型定制蛋白质时，蛋白质工程师通常会应用“达尔文式”进化原理。然而，蛋白质的实验室进化也有潜力检验进化理论并重现进化场景，从而重建假定的蛋白质中间体，并让人得以一窥“蛋白质化石”。本评论描述了应用分子进化与基础分子进化交叉领域的研究，并就基础实验与应用实验之间的协同作用如何指明在实验室中生成新蛋白质的新颖且更高效方法给出了个人观点。
