Burstein M, Legmann P
Nouv Rev Fr Hematol. 1975 Jul-Aug;15(4):425-37.
Rabbits were immunized by repeated intravenous injections of large amounts of chylomicrons plus VLDL isolated from human lipemic plasmas. The antisera were absorbed usccessively with lipoprotein free serum, HDL and LDL2. The absorbed antisera still precipitate chylomicrons and VLDL from sera of normal and hyperlipemic subjects (post-absorptive donors and fasting patients with type IV and type V hyperlipoproteinemia). By gel diffusion methods (immunoelectrophoresis and double diffusion in two dimensions), the antisera reveal a single precipitin line which stains better for lipid than for protein. This line is pronounced in VLDL-rich sera and absent in VLDL-free sera. The antisera react with chylomicrons plus VLDL delipidated by extraction with ethanol/diethyl ether; this shows that the antigenic site is an apoprotein; in contrast to apoprotein C, this apoprotein appears to be unique to the triglyceride-rich lipoproteins.
通过反复静脉注射从人高脂血症血浆中分离出的大量乳糜微粒和极低密度脂蛋白（VLDL）对兔子进行免疫。抗血清先后用无脂蛋白血清、高密度脂蛋白（HDL）和低密度脂蛋白2（LDL2）吸收。吸收后的抗血清仍能沉淀正常和高脂血症受试者（吸收后供血者以及IV型和V型高脂蛋白血症的空腹患者）血清中的乳糜微粒和VLDL。通过凝胶扩散法（免疫电泳和二维双向扩散），抗血清显示出一条单一的沉淀线，该沉淀线对脂质的染色比对蛋白质的染色更好。这条线在富含VLDL的血清中明显，在无VLDL的血清中不存在。抗血清与用乙醇/乙醚提取脱脂后的乳糜微粒加VLDL发生反应；这表明抗原位点是一种载脂蛋白；与载脂蛋白C不同，这种载脂蛋白似乎是富含甘油三酯的脂蛋白所特有的。
