Park Eun-Kee, Mak Sally K, Kültz Dietmar, Hammock Bruce D
Department of Entomology and Cancer Research Center, University of California, Davis, California, USA.
J Toxicol Environ Health A. 2007 Dec;70(24):2092-5. doi: 10.1080/15287390701551324.
Renal inner medullary collecting duct cells (mIMCD3) and human embryonic kidney cells (HEK293) were used for cytoscreening of thimerosal and mercury chloride (HgCl2). Thimerosal and HgCl2 acted in a concentration-dependent manner. In mIMCD3 cells the 24-h LC50 values for thimerosal, thiosalicylic acid, 2,2-dithiosalicylic acid, and 2-sulfobenzoic acid were 2.9, 2200, >1000, and >10,000 microM, respectively. The 24-h LC50 value for HgCl2 in mIMCD3 cells was 40 microM. In HEK293 cells, the 24-h LC50 value for thimerosal was 9.5 microM. These data demonstrate that the higher cytotoxicity produced by thimerosal on renal cells with respect to similar compounds without Hg may be related to this metal content. The present study also establishes mIMCD3 cells as a valuable model for evaluation of cytotoxicity of nephrotoxic compounds.
使用肾内髓集合管细胞（mIMCD3）和人胚肾细胞（HEK293）对硫柳汞和氯化汞（HgCl2）进行细胞筛选。硫柳汞和HgCl2呈浓度依赖性作用。在mIMCD3细胞中，硫柳汞、硫代水杨酸、2,2 - 二硫代水杨酸和2 - 磺基苯甲酸的24小时半数致死浓度（LC50）值分别为2.9、2200、>1000和>10,000微摩尔。mIMCD3细胞中HgCl2的24小时LC50值为40微摩尔。在HEK293细胞中，硫柳汞的24小时LC50值为9.5微摩尔。这些数据表明，与不含汞的类似化合物相比，硫柳汞对肾细胞产生的更高细胞毒性可能与此金属含量有关。本研究还将mIMCD3细胞确立为评估肾毒性化合物细胞毒性的有价值模型。
