Tang Hui-Fang, Mao Lian-Gen, Jiang Ruo-An, Qian Yun, Chen Ji-Qiang
Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China.
Yao Xue Xue Bao. 2007 Sep;42(9):954-8.
The aim of this study is to investigate the effect of monoammonium glycyrrhizinate (MAG) on lipopolysaccharide (LPS) -induced acute lung injury (ALI) and its anti-inflammatory mechanism in mice. All male ICR mice were randomly divided into six groups: LPS group; control group; MAG 3, 10, and 30 mg x kg(-1) groups; and dexamethasone (DXM) 5 mg x kg(-1) group. Lung dry weight and wet weight percentage and permeability were detected. Neutrophil infiltration in bronchoalveolar lavage fluid (BALF) and lung tissues was detected by cell count and morphological analysis. The levels of TNF-alpha and IL-10 in lung were detected by ELISA. MPO activity was determined followed the specification. MAG induced a decrease in lung wet weight/dry weight ratio, and significantly decreased in total leucocyte number and neutrophil percentage in the BALF, and MPO activity of lung in a dose-dependent manner. Importantly, It could up-regulate the IL-10 level and down-regulate the TNF-alpha level in the lung tissue of ALI mice. These results suggested that the protective effect of MAG in mice on LPS induced ALI was associated with the regulation of TNF-alpha/IL-10 balance, and MAG maybe a potentially treatment for ALI/ARDS.
本研究旨在探讨甘草酸单铵盐（MAG）对脂多糖（LPS）诱导的小鼠急性肺损伤（ALI）的影响及其抗炎机制。将所有雄性ICR小鼠随机分为六组：LPS组；对照组；MAG 3、10和30 mg·kg⁻¹组；以及地塞米松（DXM）5 mg·kg⁻¹组。检测肺组织干重、湿重百分比及通透性。通过细胞计数和形态学分析检测支气管肺泡灌洗液（BALF）和肺组织中的中性粒细胞浸润情况。采用ELISA法检测肺组织中TNF-α和IL-10水平。按照说明书测定MPO活性。MAG呈剂量依赖性地降低肺组织湿重/干重比值，显著减少BALF中白细胞总数和中性粒细胞百分比以及肺组织MPO活性。重要的是，它可上调ALI小鼠肺组织中IL-10水平，下调TNF-α水平。这些结果表明，MAG对小鼠LPS诱导的ALI的保护作用与调节TNF-α/IL-10平衡有关，MAG可能是ALI/ARDS的一种潜在治疗药物。
