德黑兰污水中肠球菌种类分布及万古霉素耐药基因的多重PCR检测
Distribution of enterococcal species and detection of vancomycin resistance genes by multiplex PCR in Tehran sewage.
作者信息
Rahimi Fateh, Talebi Malihe, Saifi Mahnaz, Pourshafie Mohammad R
机构信息
Dept. of Microbiology, Pasteur Institute of Iran, Tehran, Iran.
出版信息
Iran Biomed J. 2007 Jul;11(3):161-167.
BACKGROUND
Enterococci are important because of their role as the leading cause of nosocomial infections which have a significant role in the dissemination and persistence of antimicrobial resistance genes.
METHODS
In this study, we determined the distribution of enterococcal species in the sewage treatment plants in Iran. Furthermore, we improved a rapid and specific PCR method using primers (sodA and ddl genes) for identification of enterococci spp.
RESULTS AND CONCLUSION
A total number of 712 enterococci spp. were isolated and the results showed that 56%, 24%, 12%, 4%, 2%, 1% and 1% isolates were E. faecium, E. hirae, E. faecalis, E. gallinarum, E. casseliflavus, E. mundtii and other enterococcal spp., respectively. The use of species-specific PCR was in agreement with the biochemical tests. Furthermore, multiplex PCR was developed to study the presence of vancomycin resistant genes in E. faecium or E. faecalis. The multiplex PCR appeared to be a useful, rapid and specific method for detecting and discriminating genotypes for vancomycin-resistant Enterococcus.
背景
肠球菌很重要，因为它们是医院感染的主要原因，在抗菌耐药基因的传播和持续存在中起重要作用。
方法
在本研究中，我们确定了伊朗污水处理厂中肠球菌种类的分布。此外，我们改进了一种使用引物（sodA和ddl基因）的快速特异性PCR方法，用于鉴定肠球菌属。
结果与结论
共分离出712株肠球菌属，结果显示，分离株中屎肠球菌、海氏肠球菌、粪肠球菌、鹑鸡肠球菌、格氏肠球菌、蒙氏肠球菌和其他肠球菌属分别占56%、24%、12%、4%、2%、1%和1%。使用种特异性PCR与生化试验结果一致。此外，还开发了多重PCR来研究屎肠球菌或粪肠球菌中万古霉素耐药基因的存在。多重PCR似乎是一种用于检测和区分耐万古霉素肠球菌基因型的有用、快速且特异的方法。
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