Shirvani F, Behzad A, Abdollahi N, Mohkam M, Sharifian M, Esfandiar N, Fallah F
Paediatric Infections Research Centre, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Paediatric Intensive Care Department, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
New Microbes New Infect. 2021 Apr 14;41:100881. doi: 10.1016/j.nmni.2021.100881. eCollection 2021 May.
In the time span between January 2018 and September 2020, 205 patients were enrolled in a prospective cohort study at Mofid Children's Hospital. Demographic information and clinical data on all the participating children were collected and rectal swabs were performed for the sampling method. All samples were analysed so as to identify the presence of and colonization by the use of conventional biochemical tests. Resistance to vancomycin in isolates was phenotypically identified using an E-test kit and MIC value, interpreted according to the CLSI criteria. The presence of and genes, which encode the resistance to vancomycin, was screened by PCR assay. species were detected in 21.5% of rectal swab samples. (56.8%) and (43.2%) were the only species detected. species were detected in 29.3% of rectal swab samples. Out of 60 isolates, 33 (55%) were resistant to vancomycin. Moreover, was detected in 84.8% and was detected in 3% of the 33 vancomycin-resistant isolates. and species were frequently detected in the <1 year and 1-3 years age groups, respectively. Central venous access catheter and brain tumour were the main reasons for hospital admissions, 32.2% and 20.1% of total admissions, respectively. Furthermore, it must be noted that the most frequent underlying medical conditions in participating patients were esophageal atresia and hydrocephalus. The results of the present study demonstrated the necessity of determining the susceptibility of isolates to vancomycin before prescribing antibiotics.
在2018年1月至2020年9月期间，205名患者在莫菲德儿童医院参与了一项前瞻性队列研究。收集了所有参与研究儿童的人口统计学信息和临床数据，并采用直肠拭子进行采样。所有样本均通过常规生化检测进行分析，以确定是否存在[具体细菌名称1]和[具体细菌名称2]定植。使用E-test试剂盒和MIC值对[具体细菌名称1]分离株对万古霉素的耐药性进行表型鉴定，并根据CLSI标准进行解读。通过PCR检测筛选编码对万古霉素耐药的[具体基因名称1]和[具体基因名称2]基因的存在情况。在21.5%的直肠拭子样本中检测到[具体细菌名称1]。检测到的唯一[具体细菌名称1]菌种为具体菌种名称1和具体菌种名称2。在29.3%的直肠拭子样本中检测到[具体细菌名称2]。在60株[具体细菌名称2]分离株中，33株(55%)对万古霉素耐药。此外，在33株耐万古霉素的[具体细菌名称2]分离株中，84.8%检测到[具体基因名称1]，3%检测到[具体基因名称2]。[具体细菌名称1]和[具体细菌名称2]菌种分别在<1岁和1 - 3岁年龄组中频繁检测到。中心静脉通路导管和脑肿瘤是住院的主要原因，分别占总住院人数的32.2%和20.1%。此外，必须注意的是，参与研究患者中最常见的基础疾病是食管闭锁和脑积水。本研究结果表明，在使用抗生素之前确定[具体细菌名称1]分离株对万古霉素的敏感性是必要的。
