枳椇子乙酸乙酯提取物对大鼠肝脏微粒体细胞色素P450同工酶的影响

[Effects of ethyl acetate extract of Semen Hoveniae on liver microsomal cytochrome P450 isoenzyme in rat].

作者信息

Zhang Hong, Song Juan, Zhan Xin-An, Tan Ye

机构信息

Department of Pharmacy, People's Hospital, Wuhan University, Wuhan 430060, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Sep;32(18):1917-21.

PMID:18051907

Abstract

OBJECTIVE

To investigate the effects of the ethyl acetate extract of Semen Hoveniae (ESH) on liver microsomal cytochrome P450 isoenzyme in rats.

METHOD

The rats were given orally the ESH in the doses of 0.14, 0.17, 0.2 g x kg (equivalent to the crude herb) for 10 days respectively. Rat liver microsomal cytochrome P450, NADPH-Cyt C reductase, erythromycin N-demethylase (ERD), Aniline hydroxylase (ANH), aminopyrine N-demethylase (ADM) activities were quantitated by UV chromatography. The levels of mRNA expression of CYP1A1, CYP2C11, CYP2E1 and CYP3A1 were detected by semi-quantitative reverse transcripatase-polymerase chain reaction (RT-PCR).

RESULT

The cytochrome P450 content, NADPH-Cyt C reductase activities and erythromycin N-demethylase (ERD) activities were not affected. Aniline hydroxylase (ANH) activities in liver were decreased by up to35.1%; aminopyrine N-demethylase (ADM) activitiesin liver were increased by up to 42.4%. The mRNA expression of CYP1A1, CYP2C11 and CYP3A1 were found to be increased markedly.

CONCLUSION

A specific effect of ESH on liver microsomal cytochrome P450 isoenzyme in rats was observed in this investigation. ESH had various effects on liver microsomal cytochrome P450 isoenzyme.

摘要

目的

研究枳椇子乙酸乙酯提取物（ESH）对大鼠肝脏微粒体细胞色素P450同工酶的影响。

方法

将大鼠分别按0.14、0.17、0.2 g·kg（相当于生药）的剂量口服ESH，连续10天。采用紫外色谱法测定大鼠肝脏微粒体细胞色素P450、NADPH-细胞色素C还原酶、红霉素N-脱甲基酶（ERD）、苯胺羟化酶（ANH）、氨基比林N-脱甲基酶（ADM）的活性。通过半定量逆转录聚合酶链反应（RT-PCR）检测CYP1A1、CYP2C11、CYP2E1和CYP3A1的mRNA表达水平。