Zhou Dai-Xing, Yang Guang-Tian, He Xue-Xin, Liu Qiang
Department of Emergency Medicine, Tongji Hospital of Huazhong University of Science and Technology, Wuhan 430030, China.
Zhongguo Zhong Yao Za Zhi. 2007 Sep;32(18):1921-4.
To observe effects of tetrandrine (Tet) on angiotensin II (Ang II)-induced cardiomyocyte hypertrophy and the activity and expression of phosphorylated ERK1/2 (p-ERK1/2).
In the primary culture of neonatal rat cardiomyocytes, as indexes of cardiomyocyte hypertrophy, pulsation rate was measured under phase contrast microscope. Cell size was determined by cell morphology analytical system. The total protein was determined by coomassie brilliant blue and protein synthesis rate was measured by [3H]-Leucine incorporation. ERK activity was measured by immuno-precipitation. The expression of p-ERK1/2 was assessed using Western blot.
Tet can decrease Ang II-induced elevations of the pulsation rate, cell size, total protein and protein synthesis rate; inhibit the activity and expression of p-ERK1/2.
The anti-hypertrophic effect of Tet on Ang II-induced cardiomyocyte hypertrophy was associated with inhibition of ERK1/2 signaling pathway.
观察粉防己碱（Tet）对血管紧张素II（Ang II）诱导的心肌细胞肥大以及磷酸化细胞外信号调节激酶1/2（p-ERK1/2）活性和表达的影响。
在新生大鼠心肌细胞原代培养中，在相差显微镜下测量搏动率作为心肌细胞肥大指标。通过细胞形态分析系统测定细胞大小。用考马斯亮蓝法测定总蛋白，用[3H]-亮氨酸掺入法测量蛋白质合成率。通过免疫沉淀法测量ERK活性。用蛋白质印迹法评估p-ERK1/2的表达。
粉防己碱可降低Ang II诱导的搏动率、细胞大小、总蛋白和蛋白质合成率的升高；抑制p-ERK1/2的活性和表达。
粉防己碱对Ang II诱导的心肌细胞肥大的抗肥大作用与抑制ERK1/2信号通路有关。
