Moore Jeffrey C, Pollard David J, Kosjek Birgit, Devine Paul N
Department of Process Research, Merck Research Laboratories, Merck & Co., Inc. Rahway, New Jersey 07065, USA.
Acc Chem Res. 2007 Dec;40(12):1412-9. doi: 10.1021/ar700167a. Epub 2007 Dec 4.
Historically, biocatalytic ketone reductions involved the use of Baker's yeast. Within the last five years, a significant and growing number of isolated ketoreductases have become available that have rendered yeast-based reductions obsolete. The broad substrate range and exquisite selectivities of these enzymes repeatedly outperform other ketone reduction chemistries, making biocatalysis the general method of choice for ketone reductions. Presented here is a summary of our understanding of the capabilities and limitations of these enzymes.
从历史上看，生物催化酮还原反应涉及使用面包酵母。在过去五年中，大量且数量不断增加的分离酮还原酶已可获得，这使得基于酵母的还原反应过时。这些酶广泛的底物范围和出色的选择性一再优于其他酮还原化学方法，使生物催化成为酮还原反应的首选通用方法。本文总结了我们对这些酶的能力和局限性的理解。
