Lauweryns J M, Seldeslagh K A
Katholieke Universiteit Leuven, Faculteit Geneeskunde, Laboratorium voor Histopathologie, Belgium.
Regul Pept. 1991 Oct 29;36(2):183-96. doi: 10.1016/0167-0115(91)90056-m.
Calcitonin (CT) and calcitonin gene-related peptide (CGRP) are generated by alternate RNA processing from alpha and beta CT/CGRP genes. In this report, an immunocytochemical investigation was undertaken on the occurrence and distribution of immunoreactive CT as well as its colocalization with CGRP in newborn cat bronchopulmonary endocrine cells. A widespread distribution of solitary endocrine cells and neuroepithelial bodies immunostained for CT was recorded within the lung. In all animals studied, CT immunoreactivity represents a subpopulation of CGRP positive cells, while the intrapulmonary nerve fibers contain only CGRP. To the best of our knowledge, this is the first time that CT and its colocalization with CGRP have been demonstrated immunocytochemically in the cat lung. Our results indicate, that different molecular processing of both CT/CGRP genes may be represented by different patterns in the cellular immunoreactivity of the synthetized peptides.
降钙素（CT）和降钙素基因相关肽（CGRP）是由α和βCT/CGRP基因通过交替RNA加工产生的。在本报告中，我们对新生猫支气管肺内分泌细胞中免疫反应性CT的存在、分布及其与CGRP的共定位进行了免疫细胞化学研究。在肺内记录到广泛分布的单个内分泌细胞和神经上皮体对CT呈免疫染色。在所有研究的动物中，CT免疫反应性代表了CGRP阳性细胞的一个亚群，而肺内神经纤维仅含有CGRP。据我们所知，这是首次在猫肺中通过免疫细胞化学方法证实CT及其与CGRP的共定位。我们的结果表明，CT/CGRP基因的不同分子加工可能由合成肽细胞免疫反应性的不同模式所代表。
