Montavon P, Lindstrand K
Nestec Ltd., Research Centre, Vers-chez-les-Blanc, Lausanne, Switzerland.
Regul Pept. 1991 Oct 29;36(2):235-48. doi: 10.1016/0167-0115(91)90059-p.
Immunoreactivity to neuron-specific enolase (NSE), a specific neuronal marker, and calcitonin gene-related peptide (CGRP) was localized in lingual taste papillae in the pigs. Sequential staining for NSE and CGRP by an elution technique allowed the identification of neuronal subpopulations. NSE-staining revealed a large neuronal network within the subepithelial layer of all taste papillae. NSE-positive fibers then penetrated the epithelium as isolated fibers, primarily in the foliate and circumvallate papillae, or as brush-shaped units formed by a multitude of fibers, especially in the fungiform papillae and in the apical epithelium of the circumvallate papilla. Taste buds of any type of taste papillae were found to express a dense subgemmal/intragemmal NSE-positive neuronal network. CGRP-positive nerve fibers were numerous in the subepithelial layer of all three types of taste papillae. In the foliate and circumvallate papillae, these fibers penetrated the epithelium to form extragemmal and intragemmal fibers, while in the fungiforms, they concentrated almost exclusively in the taste buds as intragemmal nerve fibers. Intragemmal NSE- and CGRP-positive fiber populations were not readily distinguishable by typical neural swellings as previously observed in the rat. The NSE-positive neuronal extragemmal brushes never expressed any CGRP-like immunoreactivity. Even more surprising, fungiform taste buds, whether richly innervated by or devoid of NSE-positive intragemmal fibers, always harboured numerous intragemmal CGRP-positive fibers. Consequently, NSE is not a general neuronal marker in porcine taste papillae. Our observations also suggest that subgemmal/intragemmal NSE-positive fibers are actively involved in synaptogenesis within taste buds. NSE-positive taste bud cells were found in all three types of taste papillae. CGRP-positive taste bud cells were never observed.
神经元特异性烯醇化酶（NSE）是一种特定的神经元标志物，降钙素基因相关肽（CGRP）的免疫反应性定位于猪的舌味觉乳头中。通过洗脱技术对NSE和CGRP进行连续染色，可识别神经元亚群。NSE染色显示所有味觉乳头的上皮下层内有一个大的神经元网络。NSE阳性纤维随后作为孤立的纤维穿透上皮，主要在叶状乳头和轮廓乳头中，或作为由大量纤维形成的刷状单位，特别是在菌状乳头和轮廓乳头的顶端上皮中。发现任何类型味觉乳头的味蕾都表达密集的味蕾下/味蕾内NSE阳性神经元网络。CGRP阳性神经纤维在所有三种类型味觉乳头的上皮下层中都很多。在叶状乳头和轮廓乳头中，这些纤维穿透上皮形成味蕾外和味蕾内纤维，而在菌状乳头中，它们几乎完全集中在味蕾中作为味蕾内神经纤维。味蕾内NSE和CGRP阳性纤维群体不像先前在大鼠中观察到的那样通过典型的神经膨大容易区分。NSE阳性的味蕾外刷状物从不表达任何CGRP样免疫反应性。更令人惊讶的是，菌状味蕾，无论是否由NSE阳性味蕾内纤维丰富支配或缺乏NSE阳性味蕾内纤维，总是含有大量味蕾内CGRP阳性纤维。因此，NSE不是猪味觉乳头中的通用神经元标志物。我们的观察结果还表明，味蕾下/味蕾内NSE阳性纤维积极参与味蕾内的突触形成。在所有三种类型的味觉乳头中都发现了NSE阳性味蕾细胞。从未观察到CGRP阳性味蕾细胞。
