Miyawaki Y, Morisaki I, Tabata M J, Kurisu K, Wakisaka S
Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, Japan.
Neurosci Lett. 1996 Aug 16;214(1):29-32. doi: 10.1016/0304-3940(96)12871-8.
The distribution of calbindin D28k (CB)-like immunoreactivity (-LI) in the gustatory epithelium was examined in the adult rat. In the circumvallate and foliate papillae, CB-like immunoreactive (-IR) nerve fibers were observed in the subgemmal region, and some of these penetrated the taste buds. Two or three spindle-shaped gustatory cells displayed CB-LI in each taste bud of these lingual papillae; the immunoreactivity was restricted to the cytoplasm. In the fungiform papilla, CB-IR nerve fibers were detected in the subgemmal region, but no CB-IR cells were observed in the taste buds of the fungiform papillae. In the taste buds of the incisive papillae, many CB-IR intragemmal nerve fibers were observed, but no apparent CB-IR cells were detected. In the soft palate, CB-IR nerve fibers associated with the taste buds were also observed, but no CB-IR cells were detected in the taste buds. The present findings indicate that CB-IR gustatory cells were only localized in the taste buds in the posterior lingual papillae (circumvallate and foliate papillae), but not in the taste buds in other gustatory epithelium.
在成年大鼠中检测了味觉上皮中钙结合蛋白D28k（CB）样免疫反应性（-LI）的分布。在轮廓乳头和叶状乳头中，在味蕾下区域观察到CB样免疫反应性（-IR）神经纤维，其中一些穿透味蕾。在这些舌乳头的每个味蕾中，两到三个梭形味觉细胞显示出CB-LI；免疫反应性局限于细胞质。在菌状乳头中，在味蕾下区域检测到CB-IR神经纤维，但在菌状乳头的味蕾中未观察到CB-IR细胞。在切牙乳头的味蕾中，观察到许多CB-IR味蕾内神经纤维，但未检测到明显的CB-IR细胞。在软腭中，也观察到与味蕾相关的CB-IR神经纤维，但在味蕾中未检测到CB-IR细胞。目前的研究结果表明，CB-IR味觉细胞仅位于舌后乳头（轮廓乳头和叶状乳头）的味蕾中，而不在其他味觉上皮的味蕾中。
