聚（dG）-聚（dC）DNA在环境条件下在云母表面上看起来比聚（dA）-聚（dT）短，并且可能呈现与A相关的构象。

Poly(dG)-poly(dC) DNA appears shorter than poly(dA)-poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions.

作者信息

Borovok Natalia, Molotsky Tatiana, Ghabboun Jamal, Cohen Hezy, Porath Danny, Kotlyar Alexander

机构信息

Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel.

出版信息

FEBS Lett. 2007 Dec 22;581(30):5843-6. doi: 10.1016/j.febslet.2007.11.058. Epub 2007 Nov 29.

DOI:10.1016/j.febslet.2007.11.058

PMID:18053809

Abstract

Three types of DNA: approximately 2700 bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)-poly(dC)], approximately 2700 bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)-poly(dT)] and 2686 bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)-poly(dC) is approximately 30% shorter than that of poly(dA)-poly(dT) and the plasmid. This led us to suggest that individual poly(dG)-poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A-form of DNA in contrast to poly(dA)-poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B-form.

摘要

三种类型的DNA：约2700碱基对的聚脱氧鸟苷酸-聚脱氧胞苷酸[poly(dG)-poly(dC)]、约2700碱基对的聚脱氧腺苷酸-聚脱氧胸苷酸[poly(dA)-poly(dT)]以及2686碱基对的线性质粒pUC19被沉积在云母表面，并通过原子力显微镜成像。轮廓长度测量表明，poly(dG)-poly(dC)的平均长度比poly(dA)-poly(dT)和质粒的平均长度短约30%。这使我们推测，在环境条件下，单个poly(dG)-poly(dC)分子以一种可能与DNA的A-型相关的形式固定在云母上，这与以与DNA的B-型相关的形式固定的poly(dA)-poly(dT)和随机序列DNA形成对比。