Lapaire Olav, Volgmann Thorsten, Huang Dorothy, Hahn Sinuhe, Holzgreve Wolfgang, Zhong Xiao Yan
University of Basel, Department of Obstetrics and Gynecology, Basel, Switzerland.
Obstet Gynecol. 2007 Dec;110(6):1358-63. doi: 10.1097/01.AOG.0000295654.55971.9f.
To estimate whether potential clinical applications of cell-free fetal and total DNA in the field of noninvasive prenatal diagnosis need to be adjusted for maternal smoking status.
In this study, using 344 maternal blood samples from the second trimester of pregnancy, circulating cell-free DNA in maternal plasma samples, specific for the SRY and DYS14 loci (representing fetal DNA) and GAPDH sequence (representing total genomic DNA) were quantified by real-time polymerase chain reaction.
Fetal sex determination was 100% accurate using a combination of probes for SRY and DYS14. The levels of DYS14 and SRY detected were significantly correlated (r=0.884, P<.001). No significant difference was seen between the quantitative levels of cell-free male fetal DNA between the smoking groups and control group. Similarly, no significant difference was seen in the amount of total cell-free DNA in the study population.
In contrast to first- and second-trimester screening assays for Down syndrome, where smoking status significantly affect levels of maternal serum analytes, smoking status does not affect quantitative levels of cell-free fetal DNA or total cell-free DNA in maternal plasma.
II.
评估在无创产前诊断领域，游离胎儿DNA和总DNA的潜在临床应用是否需要根据孕妇吸烟状况进行调整。
在本研究中，使用来自妊娠中期的344份孕妇血样，通过实时聚合酶链反应对母血浆样本中针对SRY和DYS14位点（代表胎儿DNA）以及GAPDH序列（代表总基因组DNA）的循环游离DNA进行定量分析。
使用针对SRY和DYS14的探针组合进行胎儿性别鉴定，准确率达100%。检测到的DYS14和SRY水平显著相关（r = 0.884，P <.001）。吸烟组与对照组之间游离男性胎儿DNA的定量水平无显著差异。同样，研究人群中游离总DNA的量也无显著差异。
与孕早期和孕中期唐氏综合征筛查检测不同，吸烟状况会显著影响孕妇血清分析物水平，而吸烟状况并不影响母血浆中游离胎儿DNA或游离总DNA的定量水平。
II级。
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