Pidsudko Z, Kaleczyc J, Wasowicz K, Sienkiewicz W, Majewski M, Zajac W, Lakomy M
Department of Functional Morphology, University of Warmia, Olsztyn, Oczapowskiego, Olsztyn, Poland.
J Comp Pathol. 2008 Jan;138(1):23-31. doi: 10.1016/j.jcpa.2007.09.003. Epub 2007 Dec 3.
Enteric neurons are highly adaptive in their response to various pathological processes including inflammation, so the aim of this study was to describe the chemical coding of neurons in the ileal intramural ganglia in porcine proliferative enteropathy (PPE). Accordingly, juvenile Large White Polish pigs with clinically diagnosed Lawsonia intracellularis infection (PPE; n=3) and a group of uninfected controls (C; n=3) were studied. Ileal tissue from each animal was processed for dual-labelling immunofluorescence using antiserum specific for protein gene product 9.5 (PGP 9.5) in combination with antiserum to one of: vasoactive intestinal polypeptide (VIP), substance P (SP), calcitonin gene-related peptide (CGRP), somatostatin (SOM), neuropeptide Y (NPY) or galanin (GAL). In infected pigs, enteric neurons were found in ganglia located within three intramural plexuses: inner submucosal (ISP), outer submucosal (OSP) and myenteric (MP). Immunofluorescence labelling revealed increases in the number of neurons containing GAL, SOM, VIP and CGRP in pigs with PPE. Neuropeptides may therefore have an important role in the function of porcine enteric local nerve circuits under pathological conditions, when the nervous system is stressed, challenged or afflicted by disease such as PPE. However, further studies are required to determine the exact physiological relevance of the observed adaptive changes.
肠神经元对包括炎症在内的各种病理过程具有高度适应性，因此本研究的目的是描述猪增生性肠炎（PPE）回肠壁内神经节中神经元的化学编码。据此，对临床诊断为细胞内劳森菌感染的幼年大白波兰猪（PPE；n = 3）和一组未感染的对照猪（C；n = 3）进行了研究。对每只动物的回肠组织进行处理，使用针对蛋白基因产物9.5（PGP 9.5）的抗血清与针对以下之一的抗血清进行双重标记免疫荧光：血管活性肠肽（VIP）、P物质（SP）、降钙素基因相关肽（CGRP）、生长抑素（SOM）、神经肽Y（NPY）或甘丙肽（GAL）。在感染的猪中，在位于三个壁内神经丛内的神经节中发现了肠神经元：内黏膜下神经丛（ISP）、外黏膜下神经丛（OSP）和肌间神经丛（MP）。免疫荧光标记显示，PPE猪中含有GAL、SOM、VIP和CGRP的神经元数量增加。因此，当神经系统受到压力、挑战或受到诸如PPE等疾病折磨的病理条件下，神经肽可能在猪肠局部神经回路功能中发挥重要作用。然而，需要进一步研究以确定所观察到的适应性变化的确切生理相关性。
